Brain organoids represent an emerging model for studying neuronal development and connectivity. When combined with advanced microscopy systems, they enable 3D and real-time visualization of neuronal activity of early stages of human brain development. However, their density and size (≈ 2 mm) generate optical aberrations, reducing the signal-to-noise ratio at depth. To address this challenge, adaptive optics corrects these aberrations, thereby improving image quality and facilitating the analysis of neuronal signals.
This project first aims to develop adaptive optics algorithms for light-sheet microscopy, optimizing organoid imaging. It then seeks, on a longer time scale, to study the emergence and evolution of neuronal activity over several weeks. This interdisciplinary approach, at the intersection of optical microscopy, signal processing, and neuroscience, aims to develop innovative methodological tools for investigating the early stages of brain development and neuronal communication mechanisms. Focused on instrumental development in microscopy (adaptive optics) and signal analysis, the project addresses a key challenge in modern neuroscience. It also opens up applied perspectives for the study of neuronal pathologies, an active research area within our laboratory.
Antoine Hubert, bureau 3U69
École Normale Supérieure Paris-Saclay
antoine.hubert1@ens-paris-saclay.fr
To apply for this job email your details to antoine.hubert1@ens-paris-saclay.fr